The presence of certain bacteria in ready-to-use boar semen can detrimentally affect semen quality over time. Consequently, the use of contaminated semen may result in poor fertility (returns to oestrus) and endometritis (vaginal discharges) in recipient females.

Most bacteria found in contaminated ready-to-use boar semen belong to the Enterobacteriaceae family. Among them, Serratia marcescens is probably the most important due to its strong spermicidal effect and its resistance to most antimicrobials used in semen extenders.

The detection of Serratia marcescens in boar semen or laboratory equipment is usually performed using bacteriological culture procedures. However, these techniques are time consuming (48–96 hours) and detect only living bacteria.

During the past few years, PCR technology has been used to quickly and accurately detect the presence of various bacterial species in a wide variety of samples, including semen.
PCR assays are also very sensitive and detect both living and dead organisms.

At Biovet, we have developed a real-time PCR assay (qPCR) that can detect the presence of Serratia marcescens in various samples quickly (< 24 hours) and accurately (< 10 cfu/mL).

Samples may include fresh or extended semen, as well as environmental samples.

The minimum sample volume required is 10 mL.

The test is performed every day from Monday to Friday.

Results are available within 24 hours after the receipt of samples.

Please feel free to contact us for further information.


Biovet is proud to announce that a Influenza Virus Type A multispecies Antibody Test Kit, ELISA is now available!

Influenza Virus Type A multispecies Antibody Test Kit can be used in swine and several avian species. It is sensitive and specific compared to another popular commercial kit. Antibodies are detected within 7–14 days after infection and test results are available within 1.75 hours.

For further information, read our information leaflet or contact us.


Biovet now offers a new duplex qPCR assay for the simultaneous detection of porcine Circovirus type 2 (PCV2) and type 3 (PCV3).

In 2016, a new Circovirus (PCV3) was identified in the USA in cases of Porcine Dermatitis and Nephropathy Syndrome (PDNS) and abortions in sows as well as multisystemic histiolymphocytic inflammation in piglets. In these different cases, no other known virus (including PRRSV and PCV2) could be identified.

Since then, PCV3 has been identified in numerous countries, including Canada, China and several European countries. However the precise role of PCV3 remains so far to be clarified.

At Biovet, since May 2017, we offer real-time PCR (qPCR) to detect PCV3. This test will now be offered in duplex with PCV2.

The new duplex PCV2-PCV3 qPCR assay is now available and the test also includes quantification and typing of PCV2 (2a, 2b, 2d).

It will be performed once or twice a week.


Biovet now offers a New qPCR for detecting and differentiating PCV2a, PCV2b, and PCV2d

As you know, Porcine Circovirus type 2 (PCV2) has several subtypes, such as 2a and 2b.

For several years, Biovet has been putting at your disposal a qPCR allowing detection and differentiation of both subtypes.

Recently, a new subtype referred to as mutant PCV2b (mPCV2) or PCV2d has been reported.

This subtype is currently the most widespread subtype in the U.S.A. (Xiao et al, 2015).

It does not appear to be more virulent than the two other subtypes, and the available vaccines apparently offer good protection (Opriessnig et al, 2014).
In the U.S.A., PCV2d is identified by sequencing.

At Biovet, we recently developed a triplex qPCR which allows detection and differentiation of the 3 subtypes—2a, 2b and 2d.

Thanks to this PCR, we have recently identified our first case of PCV2d infection in Canadian pigs.

This new triplex qPCR 2a-2b-2d will soon replace the previous duplex 2a-2b.

Please feel free to contact us for further information.

References

  1.  Opriessnig T, Gerber PF, Xiao CT, Halbur PG, Matzinger SR, Meng XJ. Commercial PCV2a-based vaccines are effective in protecting naturally PCV2b-infected finisher pigs against experimental challenge with a 2012 mutant PCV2. Vaccine. 2014;32(34):4342-8.
  2. Opriessnig T, Xiao CT, Gerber PF, Halbur PG, Matzinger SR, and Meng XJ. Mutant USA strain of porcine circovirus type 2 (mPCV2) exhibits similar virulence to the classical PCV2a and PCV2b strains in caesarean-derived, colostrum-deprived pigs. J Gen Virol. 2014; 95(Pt 11): 2495–2503.
  3. Xiao CT, Halbur PG, Opriessnig T. Global molecular genetic analysis of porcine circovirus type 2 (PCV2) sequences confirms the presence of four main PCV2 genotypes and reveals a rapid increase of PCV2d. J Gen Virol. 2015;96(Pt 7):1830-41.