Biovet now offers a new quantitative PCR profile for the diagnosis of abortion in cattle.

In cattle, abortions normally occur at a relatively low rate of approximately 3% to 5%. Bovine abortions may result from numerous and varied causes—infectious and non-infectious. Infectious causes, however, are the most numerous and the most frequent. Among them, the most common are:

  • viruses (BVDV, IBR)
  • bacteria (Trueperella pyogenes, Salmonella spp, Streptococcus spp, Campylobacter spp., Chlamydophila spp., Coxiella burnetii, Leptospira spp., Listeria monocytogenes, Ureaplasma diversum)
  • protozoa (Neospora caninum, Tritrichomonas fœtus)
  • fungi (Aspergillus spp)

Because there are numerous possible causes of abortion, laboratory tests are essential to try to identify them. With regard to infectious agents, the detection of many of these agents using traditional methods is long and difficult. However, in recent years, faster and more sensitive molecular methods have been developed to replace conventional methods. These include, in particular, quantitative PCR (qPCR). qPCR tests are now available for many infectious agents responsible for abortions in cattle. Biovet is proud to announce that a set of tests is now available to detect several agents of bovine abortion.

The complete screening profile includes:

  • viruses: BVDV and BoHV1 (IBR)
  • bacteria: Campylobacter spp., Chlamydophila spp., Coxiella burnetii, Leptospira spp. and Ureaplasma diversum
  • protozoa: Neospora caninum and Tritrichomonas fœtus

In addition, we also offer a simplified profile, including the detection of:

  • BVDV and BoHV1 (IBR)
  • Leptospira spp
  • Neospora caninum

The samples to be submitted are fetal tissues (lung, kidney, heart, stomach contents) and placenta (placentome). For the purpose of the analysis, the tissues will be pooled in the laboratory.
The time required to complete all analyses is 4 to 5 days.

Furthermore, we recommend completing these tests with a bacteriological examination in order to detect bacteria such as Trueperella pyogenes, Salmonella spp, Streptococcus spp or Listeria monocytogenes.

Please feel free to contact us for further information.

Biovet has recently developed a multiplex real-time PCR (qPCR) test to detect and differentiate the 4 of the most frequent species of coagulase negative Staphylococci (CNS) i.e. S. chromogenes, S. epidermidis, S. simulans and S. xylosus.

The qPCR CNS test is a complementary tool to the culture and can help you to improve the control of mammary infections by more precisely identifying the CNS.

It will be performed upon request either directly from milk samples (as for PCR Staph. aureus, etc.) or from isolates obtained after culture (in this case, the costs will be added to those of the culture).
In both cases, the test will be performed every business day.

Moreover, the test will also be offered in addition to the PCR tests for the main other mammary infectious agents:

  • qPCR S. aureus + S. agalactiae + CNS
  • qPCR S. aureus + S. agalactiae + S. uberis + S. dysgalactiae + CNS
  • qPCR S. aureus + S. agalactiae + S. uberis + S. dysgalactiae + M. bovis + CNS

For further information, please feel free to contact us.

Biovet wants to promote the diagnosis of respiratory infections in cattle.

Based on the feedback from the field, it appears that attempts to diagnose these conditions often produce disappointing results.

We believe that this can be due to several factors, including:

• Samples taken too late, too few animals examined, samples from anterior rather than posterior airways
• Tests lacking sensitivity
• Lack of testing of some microorganisms

Biovet will provide you with a combination of qPCR (real-time PCR) tests to detect a wide range of pathogens including:

  1. BoHV-1 (IBR)
  2. BRSV
  3. PI3
  4. BoCV (Coronavirus)
  5. BVDV
  6. Influenza Virus D (IVD)
  7. Mycoplasma bovis
  8. Mannheimia haemolytica

The samples to be submitted are transtracheal aspirations or nasopharyngeal swabs, not simply nasal swabs.

Results will be quoted semi-quantitatively using cycle threshold (Ct) values giving an idea of the respective loads of different pathogens.

Samples with negative results will be retained for possible further testing (e.g. by Next Generation Sequencing)

The panel of 8 qPCR tests will be exceptionally offered at a low price until June 1, 2017.