Maldi-Tof petriSince we started using our new Maldi Tof bacterial identification device, we have received many questions regarding the interpretation of testing reports mentioning various species of Staphylococci, such as S. chromogenes, S. simulans, S. xylosus, S. haemolyticus, S. epidermidis, S. cohni or S. sciuri.

Before using the Maldi Tof device, we reported these species under the generic term “Staphylococcus spp” because we could not identify them accurately. In the scientific literature, these species are also often referred to as “coagulase-negative staphylococci“. About 25 species of these species have been isolated from milk samples, the most common being those cited above.

Although Staphylococci spp. are the bacterial species most frequently isolated from milk samples, they have long been considered as minor pathogens. However, recent studies suggest that different species may differ in their epidemiology and virulence. For example, S. chromogenes, S. simulans and S. xylosus would increase the somatic cell counts. S. epidermidis would be adapted to the udder, whereas S. haemolyticus would have an environmental origin.

The precise identification of these different species made possible by the use of Maldi Tof technology should ultimately help you to better understand their relative importance in your herds and adapt your control tools accordingly.

For further information, feel free to contact us.

Maldi-Tof petriBiovet has just acquired a “Maldi Biotyper Smart System”, a bacterial identification machine using MALDI TOF MS technology. This device enables extremely fast and accurate identification of bacteria as soon as isolates are obtained. This is particularly useful for bacterial species difficult or impossible to identify with traditional methods, as is the case for coagulase-negative Staphylococci.

Our home pickup service guarantees fast and safe transportation of your samples to our laboratories. Moreover, being operational 6 days out of 7, 18 hours out of 24, your samples are processed as soon as we receive them.

From now on, with our new Maldi Tof equipment, results (with precise identification) will be e-mailed to you 24 to 48 hours after receipt of the samples and will also be accessible via the Internet using our Bionet system.

Note that this improved service, even faster and more precise, will be offered at no additional cost.

No doubt this will allow you to ensure even better control of mastitis.

For further information, please feel free to contact us.

Biovet now offers a new quantitative PCR profile for the diagnosis of abortion in cattle.

In cattle, abortions normally occur at a relatively low rate of approximately 3% to 5%. Bovine abortions may result from numerous and varied causes—infectious and non-infectious. Infectious causes, however, are the most numerous and the most frequent. Among them, the most common are:

  • viruses (BVDV, IBR)
  • bacteria (Trueperella pyogenes, Salmonella spp, Streptococcus spp, Campylobacter spp., Chlamydophila spp., Coxiella burnetii, Leptospira spp., Listeria monocytogenes, Ureaplasma diversum)
  • protozoa (Neospora caninum, Tritrichomonas fœtus)
  • fungi (Aspergillus spp)

Because there are numerous possible causes of abortion, laboratory tests are essential to try to identify them. With regard to infectious agents, the detection of many of these agents using traditional methods is long and difficult. However, in recent years, faster and more sensitive molecular methods have been developed to replace conventional methods. These include, in particular, quantitative PCR (qPCR). qPCR tests are now available for many infectious agents responsible for abortions in cattle. Biovet is proud to announce that a set of tests is now available to detect several agents of bovine abortion.

The complete screening profile includes:

  • viruses: BVDV and BoHV1 (IBR)
  • bacteria: Campylobacter spp., Chlamydophila spp., Coxiella burnetii, Leptospira spp. and Ureaplasma diversum
  • protozoa: Neospora caninum and Tritrichomonas fœtus

In addition, we also offer a simplified profile, including the detection of:

  • BVDV and BoHV1 (IBR)
  • Leptospira spp
  • Neospora caninum

The samples to be submitted are fetal tissues (lung, kidney, heart, stomach contents) and placenta (placentome). For the purpose of the analysis, the tissues will be pooled in the laboratory.
The time required to complete all analyses is 4 to 5 days.

Furthermore, we recommend completing these tests with a bacteriological examination in order to detect bacteria such as Trueperella pyogenes, Salmonella spp, Streptococcus spp or Listeria monocytogenes.

Please feel free to contact us for further information.

Recurrent cases of mastitis?

What if the cause was in the bedding?

Several bacteria present in bedding can cause so-called environmental mastitis. In particular, these are Escherichia coli, Klebsiella pneumoniae and Streptococcus uberis
When such cases of mastitis occur recurrently, testing the bedding may be useful for assessing its microbiological characteristics.

Biovet offers you a “bedding profile” with counts of the following bacterial species: total mesophilic aerobic bacteria, total coliforms and Escherichia coli, Klebsiella spp., Streptococcus spp. and Staphylococcus spp.

Turnaround Time: between 3-5 days to obtain the results.

For further information, contact us.

Over the past few years, the incidence of arthritis associated with Mycoplasma hyorhinis and Mycoplasma hyosynoviae has increased significantly in the United States. Unfortunately, no reliable data are available for Canada. However, Mycoplasma hyosynoviae is common in Ontario. In Quebec, it seems that Mycoplasma hyorhinis is most common, even though cases of Mycoplasma hyosynoviae are increasingly being reported.

Biovet now offers real time PCR (qPCR) testing for Mycoplasma hyorhinis (previously available), Mycoplasma hyosynoviae and Haemophilus parasuis.

These tests are performed at least twice per week.

Tests available:

  • qPCR M. hyorhinis
  • qPCR M. hyosynoviae (NEW)
  • Combo qPCR M. hyorhinis and M. hyosynoviae
  • qPCR M. hyopneumoniae
  • Combo qPCR M. hyosynoviae and M. hyopneumoniae
  • qPCR haemophilus parasuis (NEW)

For further information, please read the technical leaflet or contact us..

Biovet will now use a real time PCR test (qPCR) for the detection of the Enzootic bovine leukosis virus (EBL). This will replace our nested PCR test.

The nested PCR method is very sensitive but long and difficult to perform (it is in fact two successive PCR)

The sensitivity of the qPCR method is close to that of the nested PCR and the specificity is equal.

QPCR is against much easier to perform and provides quantitative results.

The test is performed every day except Sunday.

Please feel free to contact us for further information.

Biovet is now performing a real time PCR (qPCR) for Mycoplasma bovis. This will replace our regular PCR test (gel based) for Mycoplasma bovis and Mycoplasma spp.

The M. bovis qPCR has a much greater sensibility than the regular PCR one (gel based).

It is also easier to perform and produces quantitative results.

The regular PCR test (gel based) for Mycoplasma spp. is still available but on special request only.

These tests are performed 3 to 5 times per week:

  • Mycoplasma bovis qPCR
  • Mycoplasma spp regular PCR (gel based)
  • Mycoplasma bovis qPCR & Mycoplasma spp. regular PCR (gel based)

Please feel free to contact us for further information.

Biovet is now using real-time qPCR instead of regular (gel based) PCR for Staphylococcus aureus, Streptococcus agalactiae, Streptococcus dysgalactiae, Streptococcus uberis and Mycoplasma bovis testing.

The qPCR offer a much higher sensibility than the regular (gel based) PCR.

They also produce quantitative results.

For further information, please feel free to contact us.

Salmonella Dublin infections has become a major concern for the Canadian cattle industry, especially veal calves but also dairies.

In presence of clinical signs compatible with salmonellosis (fever, diarrhea, pneumonia, abortion, etc.), it is important to quickly confirm (or exclude) the intervention by Salmonella spp in general and S. Dublin in particular.

At Biovet, we recently developed a procedure including a new PCR test for detecting and differentiating both Salmonella spp and Salmonella Dublin.

This procedure allows a faster and more sensitive detection of these bugs than by conventional bacteriological tests.

For more information, contact us.

Exam bacterio lait taures Graph heifer milk Two months after its implementation, here are the first results of the bacteriological testing on heifer milk samples perfrormed at Biovet.

5% of quarters were infected with Staphylococcus aureus
68% of quarters were infected with Staphylococcus spp

The distinction between Staphylococcus aureus and Staphylococcus spp is essential to limit antimicrobial treatments to the only quarters infected with Staphylococcus aureus.

The protocol used at Biovet guarantees this distinction which is not the case for some fast methods.

For further information, contact us.