Swine influenza type A virus sequencing

For several years, Biovet has collaborated with the World Organization for Animal Health (OIE) reference laboratory for influenza

For several years, Biovet has collaborated with the World Organization for Animal Health (OIE) reference laboratory for influenza A of the National Center for Foreign Animal Diseases (NCFAM) of the Canadian Food Inspection Agency (CFIA) in Winnipeg. We regularly provide them with positive samples for the swine influenza type A virus (VIA) to feed their surveillance program for this virus. Note that samples are provided anonymously.

As part of this program, Dr. Yohannes Berhane’s team is trying to isolate VIA strains and determine the composition of their genome. They are attempting to determine the gene sequences of hemagglutinin (HA) and neuraminidase (NA) by means of the Sanger sequencing method and, if possible, to obtain the complete genome sequence by the next-generation sequencing (NGS) method. Note that VIA isolates are not available for possible production of autogenous vaccines.

From the beginning of May, we will more systematically submit samples to Dr Berhane that are positive for VIA with Ct values below 30 in RT-PCR. Virus isolation is preferably performed based on lungs or nasal swabs rather than oral fluids. On the other hand, sequencing, whether by NGS or by the Sanger method, can be carried out directly from all types of samples.
For cases that will be submitted to NCAMS strictly as part of the VIA surveillance program, obviously no fees will be billed to you. In addition, we will send you a quarterly recapitulation of your cases studied, including the file #, the submission date, the holder and the identity of the subtype.

For sequencing cases that you have specifically requested, you will receive a report including the identity of the subtype and the complete HA sequence. You will be billed $50/sequencing, which includes administrative costs (file, shipment), the costs of setting up a personalized bank of VIA sequences and their possible analysis. The response time is approximately 2 weeks.

Note that the identity of the subtypes can also be obtained more quickly by the PCR method performed at Biovet at a price of $66.50 (DPOR-40137: swine influenza virus H1, H3, N1, N2 qPCR). The response time is 1 to 2 business days after obtaining a positive Influenza type A qPCR test.

Please feel free to contact us for further information.