“Suis-cides” and swine erysipelas (PCR)

A real-time PCR (RT-PCR) profile for the detection of Actinobacillus suis, Glaesserella (anc. Haemophilus) parasuis, Streptococcus suis, and

A real-time PCR (RT-PCR) profile for the detection of Actinobacillus suis, Glaesserella (anc. Haemophilus) parasuis, Streptococcus suis, and Erysipelothrix rhusiopathiae is now available from Biovet.

As their names indicate, “suis-cides” diseases are life-threatening swine (“suis”) diseases (“cides”) caused by Actinobacillus suis, Glaesserella (anc. Haemophilus) parasuis, or Streptococcus suis.

Erysipelothrix rhusiopathiae can be related to this group of bacteria as it can also be fatal, especially in young animals.

Infections caused by these microbes are usually diagnosed by their laboratory isolation and the identification of isolates by the MALDI-TOF method.

In recent years, PCRs have been developed for each of these bacteria and allow them to be detected directly from various samples.

For some infections, PCRs have been shown to be much more sensitive and faster than culture. This is particularly true for meningitis caused by Glaesserella (anc. Haemophilus) parasuis.

At Biovet, we have developed a real-time PCR profile (RT-PCR) to detect these four microbes.

SUBMITTING SAMPLES (vary depending on conditions):

  • Septicemia ( suis, A. suis, E. rhusiopathiae): filter organs such as the liver, kidney, spleen.
  • rhusiopathiae (swine erysipelas): can also be skin biopsies.
  • Pneumonia ( suis): the spleen.
  • Meningitis ( suis, G. suis): meningeal swabs
  • Polyserositis ( suis, G. suis): serous swabs and fluids from serous cavities.

TRANSMITTING RESULTS

Biovet will issue test results within 1 to 2 business days (after receiving samples).

These tests are performed Monday to Friday.

Each of these PCR tests is also available individually. To request these tests that are not on the form, indicate the name of the test in the Other Tests section.

For more information, feel free to contact us.

Références.

  1. Anonymous. Erysipelas. https://open.lib.umn.edu/swinedisease/chapter/erysipelas/
  2. Anonymous. Glaesserela parasuis. https://open.lib.umn.edu/swinedisease/chapter/haemophilus-parasuis/
  3. Anonymous. Pleuropneumonia due to Actinobacillus spp. https://open.lib.umn.edu/swinedisease/chapter/pleuropneumonia-due-to-actinobacillus-spp/
  4. Anonymous. Streptococcus suis. https://open.lib.umn.edu/swinedisease/chapter/streptococcus-suis/
  5. Giménez-Lirola LGXiao CTZavala MHalbur PGOpriessnig T. Improving ante mortem diagnosis of Erysipelothrix rhusiopathiae infection by use of oral fluids for bacterial, nucleic acid, and antibody detection. J Microbiol Methods. 2013 Feb 15;92(2):113-21.
  6. MacInnes JI, Desrosiers R. Agents of the “suis-ide diseases” of swine: Actinobacillus suis, Haemophilus parasuis, and Streptococcus suis. Can J Vet Res. 1999 Apr;63(2):83-9.
  7. Opriessnig T, Forde T, Shimoji Y. Erysipelothrix: Past, Present, and Future Directions in Vaccine Research. Front Vet Sci. 2020 Apr 15;7:174.
  8. Turni C, Pyke M,  Blackall PJ. Validation of a real-time PCR for Haemophilus parasuis. J Appl Microbiol. 2010 Apr;108(4):1323-31.
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