Salmonella spp infections are a major concern for both herd and public health.
In cattle, salmonellosis can be caused by different serotypes such as Typhimurium, Dublin, Newport, Montevideo, Muenster, Cerro, Muenchen, etc. (Gutema et al, 2019, Hong et al, 2016).
Typhimurium serotype is most common in many species.
Dublin serotype adapts well to cattle in which, unlike other serotypes, it causes persistent infections.
S. Dublin infections are particularly severe in humans.
S. Dublin strains found in Quebec are generally resistant to several families of antibiotics (multresistant strains).
Biovet provides you with diagnostic tools to detect salmonella from different samples (feces, tissues, milk, blood, food, environment).
We advocate a hybrid approach that involves combining selective enrichment (bacteriology) and real-time PCR (Goodman et al, 2017). After selective enrichment, the presence of salmonella is checked by real-time PCR.
This approach is faster and more sensitive than the bacteriological method alone.
In cattle, we currently offer 2 different PCRs:
- Salmonella 1-plex qPCR spp: can detect the presence of all salmonellas without specifying a serotype
- Salmonella 3-plex qPCR spp + S. Typhimurium + S. Dublin: can detect the presence of salmonella and determine whether or not it is Typhimurium or Dublin serotype
Given the importance of Typhimurium and Dublin serotypes, we strongly recommend the use of 3-plex qPCR which allows you to quickly know if you are dealing with a Typhimurium, Dublin or other serotype.
In the event of a positive PCR, salmonella isolation can be continued to obtain an isolate and its sensitivity to different antimicrobials can be determined using the agar diffusion method.
If the PCR is negative for Typhimurium and Dublin, then it is also possible to determine the serotype involved from the isolate.
In addition, we continue to offer salmonella research in “standard bacteriology”.
For further information, feel free to contact us.
- Goodman LB, McDonough PL, Anderson RR, Franklin-Guild RJ, Ryan JR, Perkins GA, Thachil AJ, Glaser AL, Thompson BS. Detection of Salmonella spp. in veterinary samples by combining selective enrichment and real-time PCR. J Vet Diagn Invest. 2017 Nov;29(6):844-851.
- Gutema FD, Agga GE, Abdi RD, De Zutter L, Duchateau L, Gabriël S. Prevalence and Serotype Diversity of Salmonella in Apparently Healthy Cattle: Systematic Review and Meta-Analysis of Published Studies, 2000-2017. Front Vet Sci. 2019 Apr 9;6:102.
- Hong S, Rovira A, Davies P, Ahlstrom C, Muellner P, Rendahl A, Olsen K, Bender JB, Wells S, Perez A, Alvarez J. Serotypes and Antimicrobial Resistance in Salmonella enterica Recovered from Clinical Samples from Cattle and Swine in Minnesota, 2006 to 2015. PLoS One. 2016 Dec 9;11(12):e0168016.