Nouveaux tests PCR de détection des virus influenza types A, B, C et D

Influenza viruses are divided into four types: A, B, C and D. Influenza A viruses are the most

Influenza viruses are divided into four types: A, B, C and D. Influenza A viruses are the most common and can infect humans, swine, cattle, birds and many other animal species (Henritzi and al, 2019; Zhang and al, 2019).

Influenza B viruses mainly infect humans, but they can also occasionally infect swine (Takatsy and al 1969; Ran and al, 2015; Henritzi and al, 2019).  A study of pig farms in the U.S. Midwest showed seroprevalence of 7.3% at the individual level and 38.5% at the farm level (Henritzi and al, 2018).

Influenza C viruses have also long been considered to be human-specific. However, they are occasionally also detected in swine (Guo YJ and al, 1983; Yuanji and al, 1983) and cattle (Zhang and al, 2019). A study of pig farms in Hyogo Prefecture (Yamaoka M, and al., 1991) found a seroprevalence of 19%.

Finally, influenza virus type D has been initially identified in swine (Hause et al, 2014). However, it is now considered more like a ruminant virus and has been identified in several countries on at least three continents (Liu and al, 2020). Cattle strains identified in Quebec were recently characterized (Saegerman et al, 2021).

At Biovet, we are occasionally facing influenza-like clinical problems that appear to be negative for influenza type A by PCR and ELISA antibody tests. In some of these cases, it is possible to identify porcine parainfluenza virus type 1 (PPiv-1) (Palinski and al, 2016; Park and al, 2019). However, most cases are found to be negative for these two viruses.

At Biovet, we have developed real-time PCR (RT-PCR) assays to detect and identify influenza viruses type B, C and D as well as PPiV-1 virus. These tests may be applied in cases negative for influenza type A.

SUBMITTING SAMPLES: nasal swabs, oral fluids, lungs (same as for influenza type A).

TRANSMITING RESULTS: Biovet will issue the test results within 1 to 2 business days. These tests are performed from Monday to Friday.

As part of a study of respiratory pathogens, we will make the “Influenza types B, C, D and PPiV-1 qPCR” combo free of charge when you request the combo “SRRP, M. hyopneumoniae, Influenza type A qPCR” combo. (DPOR-40139).

This offer is valid until December 31, 2021.

Pour plus d’informations, n’hésitez pas à communiquer avec nous !

Christian Savard, Director, Molecular Biology R&D
Olivier Ariel, Head of the Molecular Biology Laboratory
André Broes, Technical Support Manager


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