New qPCR test Salmonella Typhimurium

Salmonella Typhimurium (STM) is one of the main Salmonella serotypes found in humans, various animal species and animal

Salmonella Typhimurium (STM) is one of the main Salmonella serotypes found in humans, various animal species and animal products.

There are several variants of STM:

  • “typical” STMs that have the somatic (O) 1,4, [5] 12 and flagellar (H) i and 1,2 antigens (identified as i: 1,2)
  • so-called “monophasic” variants with flagellar antigens (H) that are either i (identified as i 🙂 or 1,2 (identified as -: 1,2)

Variants i: – detected for the first time in the late 1980s, they became extremely common in the 2000s. They are mainly found in pigs and humans. Some strains are resistant to several families of antibiotics.

At Biovet, we recently developed a real-time multiplex PCR (qPCR) assay that detects all STMs (“typical” and “monophasic”) and, in addition, differentiates the i: – and -: 1,2 variants
This test (qPCR STM) will replace the test used to date (this was a commercial kit). It will be used both to rapidly detect the presence of STM in various samples (manure, environment, etc.) and to characterize isolates.

The test will be performed every day.

Feel free to contact us for further information if needed.