New approach to the diagnosis of respiratory infections in cattle

We are in the winter with its respiratory problems. This year, we would like to offer you new

We are in the winter with its respiratory problems. This year, we would like to offer you new diagnostic possibilities. Indeed, it appeared that you are mainly interested in the diagnosis by PCR of viral infections and by PCR or culture for bacterial infections. To reconcile these approaches, we have created a new profile, called “respiratory viral profile PLUS” including the detection of Mycoplasma bovis in addition to that of viruses. So, here are the 4 profiles that are now available to you:

including detection of Code
1. Viral Respiratory profile BoHV1, BCoCV, BRSV, PI3, BVDV and Influenza D DBOV-20038
2. Bacterial respiratory profile Mannheimia haemolytica, Pasteurella multocida, Histophilus somni, Trueperella pyogenes and M. bovis DBOV-20039
3. Complete Respiratory profile Profils respiratoires viral et bactérien DBOV-20040
4. Viral PLUS Respiratory profile BoHV1, BCoCV, BRSV, PI3, BVDV, Influenza D + Mycoplasma bovis DBOV-20044

In addition, in order to reduce costs while maintaining good analytical sensitivity while improving diagnostic sensitivity, we suggest you resort to the use of pooled samples for the “viral respiratory profile” and “viral respiratory profile PLUS” (not for the “bacteria respiratory profile”).

Indeed, when it comes to determining whether a given contagious agent (virus, mycoplasmas) is present or not in a group of animals, it is not necessary to precisely determine the status of each of the animals concerned.

Additionally, if the individual samples are representative of the condition and the affected animals, most of these should be moderately to strongly positive.

However, the sensitivity of real-time PCR (qPCR) is such that if a pool consists of a moderately positive sample and 3 or 4 negative samples, the result of the test performed on the pool will be relatively unaffected.

Example: a pool consisting of a sample with a Ct of 28 (moderate load) and 3 or 4 negative samples (Ct> 38) will give a Ct of around 30-31.

In addition, you can request a bacteriological examination (code: DBOV-40029) completed by one or more antibiogram (s) (code: DBOV-40028) for Mannheimia haemolytica, Pasteurella multocida and Histophilus somni.

TAKING SAMPLES

Material required

  1. Paper towel or rag
  2. 30 “double sheath swabs (available at Biovet: order@biovet-inc.com)
  3. Tubes with Amies liquid transport medium (1 mL in 10 mL tube) suitable for bacteriological examinations and PCR (available at Biovet: order@biovet-inc.com)
  4. Pair of scissors
  5. Indelible marker
  6. Analysis request form
  7. Cooler with ice packs

Procedure

  1. Select 3 to 5 animals representative of the condition and at the onset of clinical signs (less than 2-3 days).
  2. Perform deep nasopharyngeal swabs on the selected animals (1 swab / animal)
    • Clean the orifice of the nasal cavities with paper towels or a cloth to limit contamination of the swabs
    • Swab the nasopharyngeal cavities as described in this video: https://www.youtube.com/watch?v=WB3luk1nQjY
  3. Cut the swab shaft to the appropriate length to allow it to be placed in a tube of transport medium.
  4. If necessary, identify the tube with the animal’s #
  5. Store samples in refrigerator (4-8 °C)
  6. Complete a request by specifying the required tests
  7. Send everything to the laboratory within 24 to 48 hours

Please feel free to contact us for further information.

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