First commercial ELISA kit for detecting APP13 antibodies *

Swinecheck APP13 major advantage is its excellent sensitivity.

Porcine pleuropneumonia (PPP), caused by Actinobacillus pleuropneumoniae (APP), leads to high economic losses in affected swine herds worldwide. A remarkable feature of APP is that its virulence greatly varies depending on the isolates. This results in clinical situations varying from subclinical infections to acute disease resulting in severe respiratory distress with high lethality.

Interestingly the virulence of a given isolate correlates quite well with the serovar in a given geographical location. So far eighteen APP serovars based on capsular polysaccharides (CPS) have been identified. Among them, serovar 13 has recently gained great importance in several European countries lying in some of them in the top 3 most important serovars isolated from clinical PPP.

Due to virulence variability the control of APP mainly focuses on the most virulent serovars. Serological testing is the most efficient tool to monitor APP infections on a herd basis. Serogroup/serovar specific assays are use to allow discriminating serotype antibodies. The most sensitive and specific assays are indirect ELISA using highly purified long chain lipopolysaccharides (LC-LPS) as antigen.

We have recently developed a LC-LPS ELISA to detect antibodies to APP13 in porcine serum samples (Swinecheck APP13 ELISA). As APP13 LC-LPS is very similar to APP7 LC-LPS, cross-reactions between these serotypes do occur in LC-LPS ELISA. However, reactions are by far stronger with the homologous antigens. The use of APP13 LC-LPS ELISA thus allows maximizing the sensitivity of detecting animals infected with this serotype.

For further information see our information leaflet or contact us.

* will not detect antibodies to North American APP13 strains which are atypical


FAST – EASY – CONVENIENT – RELIABLE

• Swinecheck® PCV2-PCV3 duplex qPCR

Swinecheck® TGEV-PEDV-PoDCV qPCR

Swinecheck® PRRSV MLV-ATP-FOS qPCR

Swinecheck® PRRSV type 1 & 2 qPCR

MAJOR ADVANTAGES

  • Fast. Results obtained in less than 90 minutes.
  • Highly sensitive. Limit of detection (LOD) around 102 copies per reaction.
  • Synthetic positive control allowing the setting of standard curves for a wide dynamic range of quantification.
  • Endogenous Control allowing avoiding false negative results.
  • Highly specific. Specificity validated in silico and with clinical specimens.
  • Convenient. Thermal conditions similar to those of other assays allowing detection of several pathogens in the same run.
  • Detailed documentation including user manual, quick protocol and certificate of analysis.

For further information read information leaflets on each kit or contact us.


Biovet is proud to announce that a Influenza Virus Type A multispecies Antibody Test Kit, ELISA is now available!

Influenza Virus Type A multispecies Antibody Test Kit can be used in swine and several avian species. It is sensitive and specific compared to another popular commercial kit. Antibodies are detected within 7–14 days after infection and test results are available within 1.75 hours.

For further information, read our information leaflet or contact us.


FAST – EASY – CONVENIENT – RELIABLE

MAJOR ADVANTAGES

  • Fast. Results obtained in less than 90 minutes.
  • Highly sensitive. Limit of detection (LOD) around 100 copies per reaction.
  • Synthetic positive control allowing the setting of standard curves for a wide dynamic range of quantification.
  • Endogenous Control allowing avoiding false negative results.
  • Highly specific. Specificity validated in silico and with clinical specimens.
  • Convenient. Thermal conditions similar to those of other assays allowing detection of several pathogens in the same run.
  • Detailed documentation including user manual, quick protocol and certificate of analysis.

To learn more, read our informations leaflets or contact us.


we have modified the composition of the dilution buffer resulting in a better homogeneity and stability.

It is still important to well homogenize it prior to be used (cf insert for use).

We are also using another type of microplate (F8) which will improve the test reproducibility.