Biovet is proud to announce that it now offers a Next-Generation DNA sequencing Microbial Test. A partnership with MiDOG, LLC which has developed those tests will be performed in their laboratory located in California.

This Microbial Test provides complete and accurate identification of bacterial and fungi pathogens to guide the design of targeted and accurate therapies.

The benefits of MiDOG are numerous:

  • Identification and quantification of ALL aerobic and anaerobic Bacteria and fungi
  • Independent of culturing
  • Detection of Antibiotic Resistances genes
  • Ambient temperature sample collection, transport, and storage
  • Turnaround time 5-8 business days

Order for free a collection kit (swab or urine in limited quantities) on our website at www.biovet.ca/boutique.

For further information, see our brochure and an example of report and visit or section www.biovet.ca/en/midog or contact us.

In partnership with


First commercial ELISA kit for detecting APP13 antibodies *

Swinecheck APP13 major advantage is its excellent sensitivity.

Porcine pleuropneumonia (PPP), caused by Actinobacillus pleuropneumoniae (APP), leads to high economic losses in affected swine herds worldwide. A remarkable feature of APP is that its virulence greatly varies depending on the isolates. This results in clinical situations varying from subclinical infections to acute disease resulting in severe respiratory distress with high lethality.

Interestingly the virulence of a given isolate correlates quite well with the serovar in a given geographical location. So far eighteen APP serovars based on capsular polysaccharides (CPS) have been identified. Among them, serovar 13 has recently gained great importance in several European countries lying in some of them in the top 3 most important serovars isolated from clinical PPP.

Due to virulence variability the control of APP mainly focuses on the most virulent serovars. Serological testing is the most efficient tool to monitor APP infections on a herd basis. Serogroup/serovar specific assays are use to allow discriminating serotype antibodies. The most sensitive and specific assays are indirect ELISA using highly purified long chain lipopolysaccharides (LC-LPS) as antigen.

We have recently developed a LC-LPS ELISA to detect antibodies to APP13 in porcine serum samples (Swinecheck APP13 ELISA). As APP13 LC-LPS is very similar to APP7 LC-LPS, cross-reactions between these serotypes do occur in LC-LPS ELISA. However, reactions are by far stronger with the homologous antigens. The use of APP13 LC-LPS ELISA thus allows maximizing the sensitivity of detecting animals infected with this serotype.

For further information see our information leaflet or contact us.

* will not detect antibodies to North American APP13 strains which are atypical


Several cases of polioencephalomyelitis caused by Porcine sapelovirus (PSV) have been recently reported in Québec herds.

Porcine sapelovirus (PSV) is an RNA virus in the family Picornaviridae. PSV is closely related to members of the Enterovirus genus and was previously known as porcine enterovirus 8 (PEV-8). Domestic and wild swine are the only known hosts for PSV.

PSV infection begins in the small intestine, where it replicates. The most common mode of transmission of PSV is fecal-oral. As the virus is hardy in the environment, fomites likely play a role in the transmission of the virus. PSV infections are commonly asymptomatic. PSV has been demonstrated in the feces of healthy swine, especially weaned piglets, in Brazil, Italy, Spain, Australia and USA.

Pathogenic strains of PSV have been reported in China, South Korea, United Kingdom, and USA. They can cause clinical syndromes including diarrhea, pneumonia, polioencephalomyelitis, and reproductive disorders (‘SMEDI syndrome’). The role of PSV as a pathogen, and more specifically as a cause of polioencephalomyelitis, is unclear. There is no specific data available on the morbidity and mortality of PSV infections in swine.

Polioencephalomyelitis induced by PSV mainly affects piglets 8 to 12 weeks old. Affected pigs demonstrate front and hind limb ataxia progressing to generalized weakness and lateral recumbency. Death may occur as soon as three to five days after the onset of clinical signs. Morbidity and mortality up to 20% have been reported. In USA, ISU, SDSU, KSU, and UMN diagnostic laboratories report 1 to 5 cases of PSV-induced polioencephalomyelitis every month.

Lesions seen with PSV-induced polioencephalomyelitis are consistent with other neurotropic viral infections, such as atypical porcine pestivirus (APPV), porcine enterovirus (PEV), porcine teschovirus (PTV), and porcine astrovirus type 3 (PAstV‐3). Polioencephalomyelitis is generally characterized as subacute, multifocal and non-suppurative.

Reverse transcriptase polymerase chain reaction (RT-PCR) is most commonly used to detect PSV in fecal samples and nervous tissues. Preferred samples for central nervous system (CNS) disease include the spinal cord and the brain. In situ hybridization has also been used to demonstrate the virus in the CNS.

A real-time RT-PCR for detecting PSV in fecal samples and nervous tissues is now available at Biovet.

The test is run on request.

Feel free to contact us for further information.

 

References

  1. Arruda PH, Arruda BL, Schwartz KJ, et al. Detection of a novel sapelovirus in central nervous tissue of pigs with polioencephalomyelitis in the USA. Transbound Emerg Dis. 2017 Apr;64(2):311-315.
  2. Leme RA, Silva DR, Lorenzetti E, et al. Longitudinal survey of Teschovirus A, Sapelovirus A, and Enterovirus G fecal excretion in suckling and weaned pigs. Braz J Microbiol. 2019;50(1):321-327.
  3. Li Y, Du L, Jin T, Cheng Y, et al. Characterization and epidemiological survey of porcine sapelovirus in China. Vet Microbiol. 2019; 232:13-21.
  4. Schock A, Gurrala R, Fuller H, et al. Investigation into an outbreak of encephalomyelitis caused by a neuroinvasive porcine sapelovirus in the United Kingdom. Vet Microbiol. 2014;172(3-4):381-9.

FAST – EASY – CONVENIENT – RELIABLE

• Swinecheck® PCV2-PCV3 duplex qPCR

Swinecheck® TGEV-PEDV-PoDCV qPCR

Swinecheck® PRRSV MLV-ATP-FOS qPCR

Swinecheck® PRRSV type 1 & 2 qPCR

MAJOR ADVANTAGES

  • Fast. Results obtained in less than 90 minutes.
  • Highly sensitive. Limit of detection (LOD) around 102 copies per reaction.
  • Synthetic positive control allowing the setting of standard curves for a wide dynamic range of quantification.
  • Endogenous Control allowing avoiding false negative results.
  • Highly specific. Specificity validated in silico and with clinical specimens.
  • Convenient. Thermal conditions similar to those of other assays allowing detection of several pathogens in the same run.
  • Detailed documentation including user manual, quick protocol and certificate of analysis.

For further information read information leaflets on each kit or contact us.


We want to inform you that we will soon offer a new real-time PCR (qPCR) test for detection of the PRRS virus. Like our previous qPCR, this test was developed in-house.
The new test will still detect and differentiate type 1 (European) and 2 (North American) PRRS viruses in the same reaction. Although only type 2 strains have been detected so far in Canada, both types are present in many countries, including the USA. Therefore, it is important to be able to detect both types of strains and to differentiate them immediately without having to use sequencing.

Within each type of PRRS virus, a wide variety of strains are constantly evolving. This characteristic represents a major challenge for diagnostic tests such as qPCR. Compared to the previous test, the new test includes more primers and probes and their composition has been diversified. These changes provide the new test with better diagnostic and analytical sensitivities.

The concentration of the virus in the samples is interesting information in some circumstances. Until now, this was expressed in TCID50 / mL. This unit is unfortunately not very intuitive. From now on, it will rather be expressed in genomic copies / mL which should be more understandable. In addition, we will also provide the Ct values (cycle threshold) as we do for our other qPCR tests.

We are confident that this new test will meet your needs even better.

Please feel free to contact us for further information.


The presence of certain bacteria in ready-to-use boar semen can detrimentally affect semen quality over time. Consequently, the use of contaminated semen may result in poor fertility (returns to oestrus) and endometritis (vaginal discharges) in recipient females.

Most bacteria found in contaminated ready-to-use boar semen belong to the Enterobacteriaceae family. Among them, Serratia marcescens is probably the most important due to its strong spermicidal effect and its resistance to most antimicrobials used in semen extenders.

The detection of Serratia marcescens in boar semen or laboratory equipment is usually performed using bacteriological culture procedures. However, these techniques are time consuming (48–96 hours) and detect only living bacteria.

During the past few years, PCR technology has been used to quickly and accurately detect the presence of various bacterial species in a wide variety of samples, including semen.
PCR assays are also very sensitive and detect both living and dead organisms.

At Biovet, we have developed a real-time PCR assay (qPCR) that can detect the presence of Serratia marcescens in various samples quickly (< 24 hours) and accurately (< 10 cfu/mL).

Samples may include fresh or extended semen, as well as environmental samples.

The minimum sample volume required is 10 mL.

The test is performed every day from Monday to Friday.

Results are available within 24 hours after the receipt of samples.

Please feel free to contact us for further information.


Dr. René Lallier, President of Biovet, is pleased to announce the appointment of Mr. Jules Lallier, BBA, MHK as Sales Manager.

Mr. Lallier started at Biovet in the summer of 2005 in various positions (customer service, receiving samples, etc.). After graduating from HEC with a Bachelor of Business Administration degree, he left St-Hyacinthe in 2011 to study at the University of Ottawa. After a few years working in the sports field in Montréal, he returned to Biovet in March 2015 to take a sales representative position in the Companion Animal and Bovine sector. We are confident that his expertise paired with his professionalism will prove to be valuable assets in his new position.

See News Release

© Photo : Patrick Roger, Photographer


Biovet is proud to announce that its Agri-Food Microbiology Laboratory has been certified by the Canadian Standards Council (SCC) as “Conforms with requirements of CAN-P-1587, CAN-P-4E (ISO/IEC 17025)”. This concerns a National Standard Method for Salmonella Enteritidis Culture and Isolation from Poultry Environmental Samples, approved by the CFIA.

Biovet operates a laboratory accredited by CFIA and SCC offering a full range of veterinary and agri-food analysis services. Our laboratories are open 6 days and operate 7 days a week. Moreover, we have an efficient pickup service providing the transport of samples in Quebec as soon as possible, even in rural areas.

For further information, please contact us.

1 CFIA : Canadian Food Inspection Agency
2 See our Scope of Accreditation on our Website (Agri-Food section).


Biovet now offers IMPROVED Digestive Profiles for canine and feline with two additional pathogens.* These real-time PCR tests for the detection of the main infectious agents of the digestive tract of the dog and the cat are performed in our laboratories enabling us to get fast results.

Each element of these profiles can be ordered individually. In addition, these new profiles can be combined with parasitology to detect even more infectious agents!
The profiles:
Canine Digestive Profiles:

* C.perfringens toxA
Campylobacter jejuni
* Canine Circovirus
Canine enteric coronavirus
Canine Parvovirus Type 2
Cryptosporidium spp
Giardia spp
Salmonella spp

 

Profil digestif Félin

* C.perfringens toxA
Campylobacter jejuni
Cryptosporidium spp
Feline Coronavirus
Feline panleukopenia virus
Giardia spp
Salmonella spp
* Rotavirus A
Toxoplasma gondii
Tritrichomonas blagburni

Sample: 5 grams of cooled fresh feces. IMPORTANT: avoid contact of feces with litter.

Delay: 48-72 hours after receipt of samples, Monday to Friday.

For further information, contact us.


Maldi-Tof petriSince we started using our new Maldi Tof bacterial identification device, we have received many questions regarding the interpretation of testing reports mentioning various species of Staphylococci, such as S. chromogenes, S. simulans, S. xylosus, S. haemolyticus, S. epidermidis, S. cohni or S. sciuri.

Before using the Maldi Tof device, we reported these species under the generic term “Staphylococcus spp” because we could not identify them accurately. In the scientific literature, these species are also often referred to as “coagulase-negative staphylococci“. About 25 species of these species have been isolated from milk samples, the most common being those cited above.

Although Staphylococci spp. are the bacterial species most frequently isolated from milk samples, they have long been considered as minor pathogens. However, recent studies suggest that different species may differ in their epidemiology and virulence. For example, S. chromogenes, S. simulans and S. xylosus would increase the somatic cell counts. S. epidermidis would be adapted to the udder, whereas S. haemolyticus would have an environmental origin.

The precise identification of these different species made possible by the use of Maldi Tof technology should ultimately help you to better understand their relative importance in your herds and adapt your control tools accordingly.

For further information, feel free to contact us.

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