The presence of certain bacteria in ready-to-use boar semen can detrimentally affect semen quality over time. Consequently, the use of contaminated semen may result in poor fertility (returns to oestrus) and endometritis (vaginal discharges) in recipient females.
Most bacteria found in contaminated ready-to-use boar semen belong to the Enterobacteriaceae family. Among them, Serratia marcescens is probably the most important due to its strong spermicidal effect and its resistance to most antimicrobials used in semen extenders.
The detection of Serratia marcescens in boar semen or laboratory equipment is usually performed using bacteriological culture procedures. However, these techniques are time consuming (48–96 hours) and detect only living bacteria.
During the past few years, PCR technology has been used to quickly and accurately detect the presence of various bacterial species in a wide variety of samples, including semen.
PCR assays are also very sensitive and detect both living and dead organisms.
At Biovet, we have developed a real-time PCR assay (qPCR) that can detect the presence of Serratia marcescens in various samples quickly (< 24 hours) and accurately (< 10 cfu/mL).
Samples may include fresh or extended semen, as well as environmental samples.
The minimum sample volume required is 10 mL.
The test is performed every day from Monday to Friday.
Results are available within 24 hours after the receipt of samples.
Please feel free to contact us for further information.