Biovet now offers a new duplex qPCR assay for the simultaneous detection of porcine Circovirus type 2 (PCV2) and type 3 (PCV3).

In 2016, a new Circovirus (PCV3) was identified in the USA in cases of Porcine Dermatitis and Nephropathy Syndrome (PDNS) and abortions in sows as well as multisystemic histiolymphocytic inflammation in piglets. In these different cases, no other known virus (including PRRSV and PCV2) could be identified.

Since then, PCV3 has been identified in numerous countries, including Canada, China and several European countries. However the precise role of PCV3 remains so far to be clarified.

At Biovet, since May 2017, we offer real-time PCR (qPCR) to detect PCV3. This test will now be offered in duplex with PCV2.

The new duplex PCV2-PCV3 qPCR assay is now available and the test also includes quantification and typing of PCV2 (2a, 2b, 2d).

It will be performed once or twice a week.

Biovet now offers a new quantitative PCR profile for the diagnosis of abortion in cattle.

In cattle, abortions normally occur at a relatively low rate of approximately 3% to 5%. Bovine abortions may result from numerous and varied causes—infectious and non-infectious. Infectious causes, however, are the most numerous and the most frequent. Among them, the most common are:

  • viruses (BVDV, IBR)
  • bacteria (Trueperella pyogenes, Salmonella spp, Streptococcus spp, Campylobacter spp., Chlamydophila spp., Coxiella burnetii, Leptospira spp., Listeria monocytogenes, Ureaplasma diversum)
  • protozoa (Neospora caninum, Tritrichomonas fœtus)
  • fungi (Aspergillus spp)

Because there are numerous possible causes of abortion, laboratory tests are essential to try to identify them. With regard to infectious agents, the detection of many of these agents using traditional methods is long and difficult. However, in recent years, faster and more sensitive molecular methods have been developed to replace conventional methods. These include, in particular, quantitative PCR (qPCR). qPCR tests are now available for many infectious agents responsible for abortions in cattle. Biovet is proud to announce that a set of tests is now available to detect several agents of bovine abortion.

The complete screening profile includes:

  • viruses: BVDV and BoHV1 (IBR)
  • bacteria: Campylobacter spp., Chlamydophila spp., Coxiella burnetii, Leptospira spp. and Ureaplasma diversum
  • protozoa: Neospora caninum and Tritrichomonas fœtus

In addition, we also offer a simplified profile, including the detection of:

  • BVDV and BoHV1 (IBR)
  • Leptospira spp
  • Neospora caninum

The samples to be submitted are fetal tissues (lung, kidney, heart, stomach contents) and placenta (placentome). For the purpose of the analysis, the tissues will be pooled in the laboratory.
The time required to complete all analyses is 4 to 5 days.

Furthermore, we recommend completing these tests with a bacteriological examination in order to detect bacteria such as Trueperella pyogenes, Salmonella spp, Streptococcus spp or Listeria monocytogenes.

Please feel free to contact us for further information.